Transcriptomics analysis of gene expression in mouse lung after PM2.5 exposure
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https://www.ncbi.nlm.nih.gov/sra/SRP168594
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资源简介：
Total RNA was isolated from mouse lung harvested at different stages using TRIzol® Reagent (Ambion). mRNA was extracted with using Dynabeads® mRNA Purification Kit (Ambion) and subjected to TURBO⢠DNase (Invitrogen) treatment at 37°C for 30 min and ethanol precipitation. Thus purified mRNA was used for RNA-Seq library construction. Libraries were directly constructed using the KAPA Stranded mRNA-Seq Kit (KAPA) and were sequenced using HiSeq2500 (Illumina) in paired-read mode, creating reads with a length of 125 bp. Overall design: Transcriptomics analysis of gene expression in mouse lung after PM2.5 exposure
创建时间：
2019-10-29
