Kidney gene expression in response to high phosphate diet

Phosphate homeostasis is a known critical function performed by the mammalian kidneys. However, changes to the transcriptome of the mammalian kidney in response to phosphate loading is unclear. The goal of this study was to examine kidney gene expression in response to high phosphate diet, compared to normal phosphate diet. RNA-seq was performed on the kidneys of C57BL/6J mice who were fed a high phosphate diet and in mice who received a normal phosphate diet for three days. C57Bl/6J mice were placed on experimental diets (Envigo) containing normal phosphate (0.6%) or high phosphate (1.2%) diets for three days (n=4 in each group). mRNA was prepared from kidney cortex samples acquired on day 3