Transcriptional analysis on the impact of anti-PD-L1 mAb on murine lung tumor sorted myeloid populations.

Targeted gene expression on Lewis lung carcinoma (LLC) sorted MHC-IIlow macrophages (M2), Ly6Chigh inflammatory (IM) and Ly6Clow residential (RM) monocytes to investigate the effect of anti-PD-L1 mAb on specific myeloid subsets in the lung tumor microenvironment performed using the nCounter Myeloid Innate Immunity Panel by Nanostring . C57BL/6 mice were challenged intravenously with LLC cells and treated every 3 days with anti-PD-L1 or isotype control mAbs for 4 times in total. On week three lungs were isolated and MHC-IIlow macrophages, Ly6Chigh and Ly6Clow monocytes were sorted for RNA isolation (3-4 biological replicates).