KRT14 knockdown enhances cisplatin-resistance through upregulating LRP11 expression in ovarian cancer

Ovarian cancer is a highly malignant gynecological tumor. Multidrug resistance (MDR) in tumors is a major reason for chemotherapy failure. Keratin 14 (KRT14), a member of the keratin family, plays a significant role across various cancers. Elevated KRT14 expression has been observed in ovarian cancer tissues compared to normal ovarian tissues, and this differential expression is associated with poorer progression-free survival in patients undergoing platinum and taxol-based chemotherapy. Furthermore, KRT14 expression is heightened in migratory ovarian cancer cells, where it plays a crucial role at the invasive interface, facilitating ovarian cancer cell invasion. The role of KRT14 in regulating MDR is not known. In this study, we aimed to investigate the effects and mechanisms of KRT14 in human cisplatin-resistant ovarian cancer cell lines SK-OV-3/DDP and A2780/DDP. Overall design: SK-OV-3/DDP and A2780/DDP cells were transfected with small interfering RNA (siRNA) targeting KRT14 (siKRT14) or negative control siRNA (siNC) for 24 h and then cells were harvested for RNA sequencing.