<p>Performance of the four aligners PymiRa, Chimira, miRDeep2, and Bowtie2 in the 10 million read simulated dataset. A) Number of mature miRNAs identified and their Root Mean Square Error (RMSE). B) RMSE of the miRNAs identified by each aligner in the top 600 ‘over-expressed’ simulated miRNAs. Of the real miRNA sequences, 600 of these were randomly selected to be given increased counts to simulate a disease model where there is miRNA dysregulation (i.e., over-expression of specific miRNAs).</p>

Performance of the four aligners PymiRa, Chimira, miRDeep2, and Bowtie2 in the 10 million read simulated dataset. A) Number of mature miRNAs identified and their Root Mean Square Error (RMSE). B) RMSE of the miRNAs identified by each aligner in the top 600 ‘over-expressed’ simulated miRNAs. Of the real miRNA sequences, 600 of these were randomly selected to be given increased counts to simulate a disease model where there is miRNA dysregulation (i.e., over-expression of specific miRNAs).