Quantitation of Poly Unsaturated Fatty Acids in the OPPERA Study

This is a set of quantitative LC-MS/MS data acquired on extracted human red blood cells form the OPPERA study. An assortment of Omega 3 and 6 fatty acids were monitored as well as their corresponding lipid mediators: Eicosapentaenoic acid, α-linolenic acid, Docosahexaenoic acid, Arachidonic acid, Linolenic acid, Resolvin 1, Prostaglandin E2, Protectin D1, Maresin 1, Maresin 2, 18-HEPE, 9-HODE, and 17-HDHA. The analysis was performed in 7 different batches over the course of 3 weeks on a Thermo TSQ Vantage triple quadrupole mass spectrometer coupled to a Waters Acquity UPLC. The purpose of this analysis was to relate the amount of free poly unsaturated fatty acids to the absence or presence of pain conditions in humans.