Location and collection number of the analyzed crayfish species.

Host species of each of the analyzed populations, i.e., Austropotamobius pallipes, Procambarus clarkii and Pacifastacus leniusculus sampled from the Real Jardín Botánico crayfish collection, including corresponding collection codes and year. The location of the host species and the nearest invasive alien species (IAS) is indicated (the (X) indicates IAS were not checked in the surroundings). Result of the microscopy analysis and diagnostic PCR with A. astaci primers 42 and 640 are also provided: (+) indicates visible signs of disease were observed or the PCR fragment was successfully amplified and the (-) indicates no signs of disease were observed or (--) the PCR was not amplified in the respective analyses. The mitochondrial haplotype of some of the analyzed specimens is also provided (in bold).