RNA-seq analysis of the jojoba leaves

Whole transcriptome shotgun sequencing (RNA-seq), a high-throughput transcriptome sequencing technology based on the next generation sequencing technology. Through RNA-seq, A large number of mRNA sequences can be obtained quickly and at a lower cost by assembling the huge amounts of sequencing reads, and this is particularly important for species lacking genomic information, such as jojoba. At the same time, by counting the number of reads mapping to specific gene sequences in the genome, RNA-seq can provide the accurate expression level of all genes in a particular tissue or under specific treatment condition readily. Numerous studies using RNA-seq technique have been conducted to establish high-quality reference transcriptome sequence , identified key enzymes in metabolic pathways related to the synthesis of phytochemical constituents, or identify differentially expressed genes under environmental stress in crops and many plant species with high economic, ornamental, or ecological value. However, the transcriptome sequencing of jojoba has not been reported by far, despite its important economic and ecological value.In the present study, we established a general jojoba transcriptome dataset by using RNA-seq.