Time-series proteomics and phosphoproteomics analysis of human airway epithelial cells in the absence or presence of a specific IKK inhibitor

Epithelial-mesenchymal transition (EMT) plays a critical role in airway injury, repair, and structural remodeling. Although NFkB/RelA subnit is involved in late EMT-associated gene expression, RelA translocation is occurs later than early phases of IκB kinase (IKK)-depenedent gene expression. To investigate the hypothesis that IKK plays an independent mechanism in TGF-induced EMT, we conducted time-series proteomics and phosphoproteomics analysis of human airway epithelial cells in the absence or presence of a specific IKK inhibitor, BMS -345541.