CIL:35378, Homo sapiens, cevical carcinoma. In Cell Image Library
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HeLa cells were metaphase-arrested by a 3hr treatment with colcemid, the cells mechanically broken by passage through a 22-gauge needle and exposed to hypotonic 75 mM KCl for 10 min. Samples were fixed with 3:1 methanol:acetic acid, squashed in 45% acetic acid, transferred to a formvar coated grid, critical point dried, and imaged with the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic microscopy of chromosomes. Meth Cell Biol 22:77-96.
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UC San Diego Library Digital Collections
创建时间：
2021-04-15
