Limiting dilution culture of 100% GFPpos TT-specific B cells.
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Indicated is the total number of clones isolated and at which cell density they were seeded. One 96 well plate was used for each condition (1, 5 or 10 cell per well). Wells contained 2500 CD40L-expressing L cells, IL-2 and IL-4. ¼ to ½ of the medium was replaced twice a week with fresh cytokines and 5000 L cells. N.D. is not determined.
创建时间：
2015-12-02
