Table 2 in Description of Alvania wangi Xu, Qi & Kong, sp. nov. (Mollusca, Gastropoda, Littorinimorpha, Rissoidae) from the East China Sea
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Table 2. Target gene, primer details and PCR conditions (temperature, time, and number of cycles) applied in the present study.
GenePrimerSequence 5 ’-3’ReferencePCR conditions28S28SDKFF: GATCGGACGAGATTACCCGCTGAAStrong et al. 201194 °C (7'), 58 °C (1'), 72 °C (2') [x1]; 94 °C (1'), 52 °C (1'), 72 °C (2') [x35]; 94 °C (1'), 52 °C (1'), 72 °C (7') [x1]LSU 1600RR: AGCGCCATCCATTTTCAGGWilliams et al. 200316S16SARisF: TGCCTGTTTAGCAAAAACATCriscione and Ponder 201394 °C (5'), 52 °C (30 ’’), 72 °C (1') [x1]; 94 °C (30 ’’), 52 °C (30 ’’), 72 °C (1') [x40]; 94 °C (30 ’’), 52 °C (30 ’’), 72 °C (7') [x1]16SBRisR: CCGGTCTGAACTCAGATCATGT
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2025-04-04
