Dars2 is involved in mRNA splicing in mouse bone marrow hematopoietic stem and progenitor cells

Deletion of Dars2 in HSPCs largely impairs spliceosome function and suppresses the protein expression of Srsf3.We performed high-coverage mRNA-seq on lin- ckit+ (LK) cells from both WT and Dars2-KO mice, respectively, to characterize genome-wide alternative splicing (AS) events affected in Dars2-KO HSPCs. Globally, 3970 AS events were significantly changed by loss of Dars2 in HSPCs, including 2431 events in skipped exons (SEs), 516 events in retained introns (RIs), 375 events in mutually exclusive exons (MXEs), 340 events in alternative 3' splice sites (A3SSs) and 308 events in alternative 5' splice sites (A5SSs) Overall design: Comparative gene expression profiling analysis and alternative splicing analysis of RNA-seq data for Dars2 WT and Dars2 KO lineage negative and ckit positive cells.