Comparative whole-genome transcriptome analysis in renal cell populations reveals high tissue specificity of MAPK/ERK-targets in embryonic kidney

We report here bulk RNA sequencing results of control and MAPK/ERK-deficient ureteric bud (UB) epithelium and nephron progenitor cells (NP) in the developing mouse kidney. RNA isolated from 4 biological replicates for UB and 3 biological replicates for NP was subjected to the library preparation done using NuGen Ovation Solo. Sequencing with NextSeq was performed at BIDGEN DNA Sequencing, after data processing produced 1004 (UB) and 5053 (NP) differentially expressed genes with a statistical cutoff of Padj<0.05 and a magnitude threshold of ¦log2foldchange¦=1 . Overall design: Examination of differential gene expressions in two different embryonic kidney tissues: wild type and MAPK/ERK-deficient UB and wild type and MAPK/ERK-deficient NP cells