Isolation and genetic characterization of a novel Kevo orbivirus and a strain of Mobuck virus from Ochlerotatus communis mosquitoes in Finland

Supplementary Material for 'Isolation and genetic characterization of a novel Kevo orbivirus and a strain of Mobuck virus from <i>Ochlerotatus communis</i> mosquitoes in Finland' as described in <i>Journal of General Virology.</i><br>This dataset contains 5 jpeg images that depict the phylogenetical trees based on VP1-NS3 nucleotide sequences (Figures S3-S5) and VP4-N3 amino acid trees (Figures S1-S2). The GenBank_accession_codes.xlsx file contains three tables where all GenBank accession numbers used in the study are provided. The Matrix_tables_final.xlsx file contains three tables that depict VP3(T2) amino acids, VP3(T2) nucleotide and VP1 percent identities of selected orbiviruses. The pdf file contains Supplementary Table 4 that contains information regarding the pools.<b>Figure S1. </b>Maximum likelihood trees based on VP4, VP5 and VP6 amino acid sequences. EHDV-1, BTV, AHSV-1, WALV, EUBV and ORUV used as outgroup. Coding sequences (CDS) of KEVOOV and MBV Ilomantsi strain were used. Bootstrap values over 70 are shown. KEVOOV is highlighted in orange and MBV Ilomantsi strain in blue.<b>Figure S2. </b>Maximum likelihood trees based on NS1, NS2 and NS3 amino acid sequences. EHDV-1, BTV, AHSV-1, WALV, EUBV and ORUV used as outgroup. Coding sequences (CDS) of KEVOOV and MBV Ilomantsi strain were used. Bootstrap values over 70 are shown. KEVOOV is highlighted in orange and MBV Ilomantsi strain in blue.<b>Figure S3. </b>Nucleotide maximum likelihood trees based on VP1, OC1, T2, and VP7 sequences. EHDV-1, BTV, AHSV-1, WALV, EUBV and ORUV were used as outgroups. Coding sequences (CDS) of KEVOOV and MBV Ilomantsi strain were used. Bootstrap values over 70 are shown. KEVOOV is highlighted in orange and MBV Ilomantsi strain in blue.<b>Figure S4. </b>Nucleotide maximum likelihood trees based on VP4-VP6 sequences. EHDV-1, BTV, AHSV-1, WALV, EUBV and ORUV used as outgroup. Coding sequences (CDS) of KEVOOV and MBV Ilomantsi strain were used. Bootstrap values over 70 are shown. KEVOOV is highlighted in orange and MBV Ilomantsi strain in blue.<b>Figure S5. </b>Nucleotide maximum likelihood trees based on NS1-NS3. EHDV-1, BTV, AHSV-1, WALV, EUBV and ORUV used as outgroup. Coding sequences (CDS) of KEVOOV and MBV Ilomantsi strain were used. Bootstrap values over 70 are shown. KEVOOV is highlighted in orange and MBV Ilomantsi strain in blue.<b>Table S1. </b>Percent Identity Matrix of PHSV clade orbiviruses, EHDV-1, BTV, AHSV-1, WALV, EUBV and ORUV virus based on VP3(T2) amino acid sequences. Values over 83% are highlighted in orange. (Can be found in the Matrix_table_final.xlsx file, sheet VP3 aa matrix).<b>Table S2. </b>Percent Identity Matrix of PHSV clade orbiviruses, EHDV-1, BTV, AHSV-1, WALV, EUBV and ORUV based on VP3(T2) nucleotide sequences. Values over 76% are highlighted in orange. (Can be found in the Matrix_table_final.xlsx file, sheet VP3 nt matrix).<b>Table S3. </b>Percent Identity Matrix of PHSV clade orbiviruses, EHDV-1, BTV, AHSV-1, WALV, EUBV and ORUV based on VP1 amino acid. Values over 74% are highlighted in orange. (Can be found in the Matrix_table_final.xlsx file, sheet VP1 aa matrix).<b>Table S4. </b>Details of the 11 superpools which were constructed by species from the 94 previously studied mosquito pools.<b>Table S5. </b>GenBank nucleotide and protein accession numbers used in the phylogenetic trees and recombination detection analysis. (Can be found in the GenBank_accession_codes.xlsx file).