The effect of fructose vs glucose supplementation on stable AKR1B1 knockdown in A549 lung cancer cells

To investigate the cytotoxic effect of AKR1B1 depletion, we induced stable AKR1B1 knockdown via shRNA under different growth conditions (glucose vs fructose) We then performed gene expression profiling analysis using data obtained from RNA-seq of control (non-targeting shRNA) or shAKR1B1 A549 cells 5 days after shRNA delivery. Overall design: Comparative gene expression profiling analysis of RNA-seq data for A549 control or shAKR1B1 cells, maintained in media containing either 5mM glucose or fructose.