Total proteome of germinative and dormant axillary buds of the sugarcane cultivar RB867515

Sugarcane is the raw material for the production of ethanol and sugar, and its by-products are used in cogeneration of energy in addition to various industrial processes. The cane field establishment is dependent of the axillary buds sprouting. The present study aimed to catalog the total proteome of germinative axillary bud and dormant axillary buds, and to identify important budding proteins, which are differentially expressed in these types of axillary buds. The proteome was obtained by extraction of total proteins using the TCA acetone method adapted for axillary buds. The experiment was carried out in biological triplicate. Protein samples were submitted to denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and stained with comassie blue. Each lane of the polyacrylamide gel was fractionated (11 slice) and treated for purification and isolation of the polypeptides. Tryptic digestion was performed on each fragment with subsequent UPLC separation (Nano Acquity). The polypeptides were analyzed on Waters® Micromass® ESI-Q-Tof micro ™ mass spectrometer. Protein samples from the germinative and dormant axillary buds showed distinct bands patterns in the SDS-PAGE gel. Mass spectrometry data were analyzed by MASCOT tool (http://www.matrixscience.com).