Hippocampus and visceral adipose tissues cellular landscape profiling to characterize the effects of obesity-amyloidosis comorbidity [scRNA-seq]

To explore the link between Alzheimer's disease (AD) and obisity, we profiled the transcriptional changes of the co-morbidites as well as indivitual morbidities on brain, immune and adipose tissue, at the single cell level. We observed effects of both morbidities, including AD-associated alterations in microglia and astrocytes in AD mice, and HFD-associated transcriptional changes in teh brain and the adipose. See details in: doi: https://doi.org/10.1101/2022.02.05.479219 We preforemed single nuceli RNA-seq sequencing of the hippocampus and adipose tissue of WT and AD-model (5xFAD) mice maintained on control or high fat diet, in 7-9 months old mice.