Transcription and cap trimethylation of a nematode spliced leader RNA in a cell-free system.
收藏PubMed Central2026-05-16 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC53335/
下载链接
链接失效反馈官方服务：
资源简介：
Maturation of a fraction of mRNAs in nematodes involves the acquisition of a common 5' terminal spliced leader sequence derived from a nonpolyadenylylated spliced leader RNA by trans splicing. We have developed a cell-free system prepared from Ascaris lumbricoides embryos that accurately and efficiently synthesized the spliced leader RNA of A. lumbricoides. Transcription of the spliced leader RNA was catalyzed by RNA polymerase II, and the majority of the spliced leader RNAs synthesized in vitro possessed a trimethylguanosine cap structure identical to that found on in vivo-synthesized spliced leader RNA. IMAGES:
提供机构：
National Academy of Sciences
