A novel mouse stem cell established in combination of Activin A and LIF

We reported the ALSCs derivation from E3.5 blastocysts and E6.5 epiblasts cultured in basic N2B27 medium supplemented with Acitivin A and LIF. Activin A subtitutes NODAL signaling in vivo and is an important factor for self-renewal. LIF is extracted from feeder and have an impact on sustaining pluripotency of ESCs. We combined the two factors and finding AL medium could sustaining a novel mouse embryonic stem cells. Furthermore, EpiSCs are also discovered that able to convert to rESCs by culturing in AL medium for several passages, which illustrates that AL medium not only support establishment of pluripotent stem cells from embryos in vivo but also could convert EpiSCs to rESCs. Then, based on multiple analyses of pluripotency, we performed RNA-seq of ALSCs to exploring the differences of ALSCs compared to EpiSCs and ESCs respctively. RNA-seq data showed ALSCs is different with EpiSCs or ESCs. Teratoma test proved ALSCs could contribute to three germ layers in vivo and embryonic body analysis testify ALSCs could differentiate to three germ layers like cells in vitro. Overall design: 3 replicates of mRNA of ALSCs. The control group named AFSCs and have been submitted in GSE130060.