Concentrations of selected analytes in mainstream smoke of commercial cigarettes to which 13C-labeled and unlabeled sugars were added.

[13C]-isotope labeled sugars were added to cigarettes. Different concentrations, sugars (fructose, glucose, sucrose, and a mixture) were tested with eight cigarette brands, with different design characteristics such as length and filter ventilation and sugar content. Cigarettes were machine-smoked (using ISO and WHO Intense regimes), and the resulting samples were subjected to untargeted and targeted analyses (for volatile organic compounds (VOCs), polycyclic aromatic hydrocarbons (PAHs), aldehydes, tobacco-specific nitrosamines (TSNAs) and nicotine).