S4 Datasheet -

(A) Shown are the fold changes in dilp2, dilp3, or dilp5 mRNA amounts collected from the heads of flies that were unexposed or dead-exposed for 14 days. (B) The values shown are the change in the anti-Dilp3 antibody staining intensity of the MNC in the brains of flies that were unexposed or dead-exposed for 14 days minus the background fluorescence for each brain analyzed. (C–F) The first column is the age of the animals in days, while each subsequent column contains the survivorship values calculated for the fly population indicated at the top. The genotypes of the flies used were: (C) w- Dahomey (control) or dilp2-3,5 triple mutant flies, (D) w1118 (control) or dilp2 mutant flies, (E) w1118 (control) or dilp3 mutant flies, and (F) w1118 (control) or dilp5 mutant flies. (G–I) The values shown are the change in the anti-Dilp3 antibody staining intensity of the MNC, minus the background fluorescence for each brain analyzed. The treatments consisted of the following: (G) R4d-GAL4 x UAS-Chrimson flies kept in the dark (control) or given an acute (2 days) red light exposure, (H) R4d-GAL4 x UAS-Chrimson flies kept in the dark (control) or given a chronic (14 days) red light exposure, and (I) R4d-GAL4 x w1118 flies kept in the dark (control) or given a chronic (14 days) red light exposure. (XLSX)