Mouse ES cells are a powerful platform to analyze celullar differentiation in vitro. ES cells can be mutagenized by various methods such as a chemical mutagenesis using ENU. To investigate a mutationa
To confirme the influenece of introduction of T29I mutation in PRSS1 to the gene expression profile of ES cells, we isolated mRNA from wild-type ES cells or mutation-introduced ES cells. This data sho
PCR products were obtained from each target loci using genomic DNA from ENU-mutagenized mouse ES cells. Subsequently, PCR products are pooled and subjected to Illumina library preparation. The library
Expression data from ES cell exposed to chemicals Overall design: RNA from mouse ES cells was extracted and processed for hybridization on Affymetrix microarrays.
