Translation efficiency in the pancreatic tumor in response to ketogenic diet and targeting eIF4E phosphorylation

To investigate the translatome in the pancreatic tumor inresponse to ketogenidc diet and the combination of ketogenic diet with an inhibitor targeting eIF4E phosphorylation. We generated pancreatic tumor xenograftmice model, treated mice with regular chow, ketogenic diet or ketogenic diet with eFT508, an inhibitor targeting eIF4E phosphorylation. Then we performed Poly-RNAseq on the tumor isolated from mice upon different treatments. We isolated the tumor isolated from T3M4 injected nude mice on different treatments. We then seperated highly translated mRNA and low translated mRNA using sucrose gradient and perform comparative gene expression profiling analysis of RNA-seq data for those high tanslated and low translated mRNA.