Ag-specific IgG isotype responses in mice vaccinated with different vaccine regimens in short-term protected group (ten days post boost) before and 3 months after L. donovani challenge infection.

Ten days after final immunization and 3 months after challenge infection, blood serum samples were collected and assayed for IgG2a, IgG1, and IgG2a:IgG1 by ELISA. The results are shown as the mean absorbance values ± S.E. of five individual mice per group, representative of two independent experiments with similar results. p values were calculated using one-way ANOVA and Tukey's multiple comparison test.aSignificantly higher than gp63 DNA either free or in presence of CpG (pbSignificantly higher than DNA-prime/Protein-boost (pcSignificantly higher than gp63 DNA (p