Drosophila in vivo screening for phagocyte factors protective against L. amazonensis infection.

Gene knockdown was targeted to hemocytes by crossing a fly line carrying the Hml(Δ)-Gal4-driver to UAS-RNAi-expressing flies. Croquemort and draper mutant lines were also analyzed, as indicated. The 3rd column presents the percentage of assays where the survival of the experimentally-infected flies was significantly reduced compared to PBS-injected RNAi flies and to control amastigote-infected flies (pFig 3.