Effects of abalone visceral polypeptide on intestinal microbiota of mice investigated by Illumina Miseq sequencing

In this study, the experimental mices were fed via intragastric administration with polypeptide extracted from abalone viscera, and the intestinal microbiota and its changes were analyzed by Illumina Miseq sequencing. The results showed that the dominant bacterial phyla in intestine of mice were Bacteroides, accounting for 69.1% of the total sequence, followed by Firmicutes and Proteobacteria, accounting for 23.5% and 5.0% of the total sequences, respectively. The Proteobacteria was dominated by δ-Proteobacteria. On the genus level, the most dominant group was norank_f_Bacteroidales_S24-7_group, accounting for 30.33%, followed by Alloprevotella and Bacteroides, accounting for 12.47 and 9.8%, respectively. After the intragastric administration of abalone polypeptide, the abundance of 8 bacterial genera in the intestine of mice increased, in which the genera Parabcteroides, unclassified f__Ruminococcaceae and Marvinbryantia increased significantly (p<0.05). The abundance of 8 genera decreased, in which the genera norank_f_Lachnospiraceae and Anaeroplasma decreased significantly (p<0.05).