Comparative transcriptomic analysis of THP-1 derived macrophages infected with Mycobacterium tuberculosis H37Rv, H37Ra and BCG

In this study, RNA sequencing (Transcriptome sequencing) was employed to investigate the global transcriptome changes in the macrophages during the different strains of M.tb infection. THP-1 cells derived macrophages were exposed to the virulent M.tb strain H37Rv (Rv) or the avirulent M.tb strain H37Ra (Ra), and the M.tb BCG vaccine strain was used as a control. The cDNA libraries were prepared from M.tb infected macrophages and then sequenced. Overall design: The experiments were divided into four groups include; control, H37Rv, H37Ra, and BCG infected groups respectively. There are three samples in each group and 12 samples in total