Comparision of gene expression in adipose progenitors(AP) relative to adipose tissue(AT)

To identify of candidate transcriptional regulators of AP function, microarray was utilized to analyze gene expression in freshly isolated AP from stromal-vascular fractions relative to whole adipose tissue (AT) from the same mouse. We utilized negative selection strategy, utilizing antibody coated magnetic beads to deplete hematopoietic cells (lineage+) and CD31+ endothelial cells from the stromal-vascular fraction after enzymatic dissociation of AT depots, which yielded putative APs from the inguinal (SAT) and perigonadal (VAT) depots of several mice in approximately 30 minutes.