The soil bacterium Lysobacter capsici attaches to the nematode surface, and triggers induced systemic resistance in barley, impairing the invasion of root-lesion nematodes

Root-lesion nematodes pose a global threat to crop yield and stability, with their broad host range presenting a formidable challenge for effective management. In this study, we investigated the role of the nematode-attached bacterium Lysobacter capsici in mitigating the invasion of Pratylenchus penetrans into spring barley roots. Using a split-root system, we demonstrated that the cuticle-attached bacterium significantly reduced nematode invasion by 75% to 77% as compared to the nematodes without L. capsici. This reduction was predominantly due to the induction of systemic defense in the plants, and partially due to the bacterium's local antagonism in contact with the nematode.Compared to the nematodes without L. capsici, the reduced root invasion of P. penetrans with attached bacteria differed over time and was particularly observed at 48- and 72 hours post-inoculation. Notably, the reduction in nematode invasion was more pronounced when L. capsici cells were attached to the nematode ..., Two datasets from invasion of endoparasitic nematodes into roots as affected by a bacterial strain of Lysobacter capsici that attaches to the surface of the nematodes., , # The soil bacterium Lysobacter capsici attaches to the nematode surface, and triggers induced systemic resistance in barley, impairing the invasion of root-lesion nematodes

[https://doi.org/10.5061/dryad.qbzkh18t6 ](https://doi.org/10.5061/dryad.qbzkh18t6)

## Description of the data and file structure

***In vitro***** split-root system to study *Lysobacter capsici* effects on root invasion by *P. penetrans***

We incubated 20000 surface-sterilized nematodes in 5 ml of bacterial suspension (OD600=0.01) overnight at 22°C on a shaker at 150 rpm. As a control, we incubated surface-sterilized nematodes in sterile tap water under the same conditions. After incubation, we washed nematodes on a 5-µm CellTrics sieve with 10 ml of sterile tap water to remove loosely attached bacterial cells. We inoculated one side of the barley split-root system with 200 nematodes with or without attached bacterial cells. As a control for plant-mediated effects of the bacteria, we inoculated 200 surface-steri...