Supplementary Data S3
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Supplementary Data S3 of the manuscript "<b>Hypoxia leads to reduced mito-nuclear gene expression and increased mtDNA transcriptional pausing in human cells</b>" by Shtolz et al.The data was used to generate Fig. 3d-g within the manuscriptDNA qPCR data used in the study to measure copy number. The data was generated as described in the Materials and Methods section:To quantify the relative mtDNA copy number, qPCR (Bio-Rad CFX96) was performed using SYBR Green (Applied Biosystems, Thermo Fisher Scientific) on genomic DNA samples with two primer pairs targeted to amplify two nDNA genes (<i>JUN</i> and either <i>ANKRD37 </i>or <i>ACTB</i>) and four primer pairs targeted to amplify four mtDNA fragments selected to represent four mtDNA regions (Table 1). Each sample and amplicon combination were analyzed in triplicate for four biological replicates of hypoxia experiments per cell line.<br>
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figshare
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2025-10-03
