Bulk RNA-seq on paws of collagen-induced arthritis (CIA) mouse model at different disease stages

The goal of this experiment is to profile longitudinal transcriptome changes during the development of murine arthritis in CIA mouse model. Male DBA1 mice (age between 10 to 12 weeks) were used in this study. CIA was induced by two immunizations with Type II collagen in Complete Freund’s adjuvant (CFA) at day 0, and with Type II collagen in Incomplete Freund’s adjuvant (IFA) at day 21. Bulk RNA-seq were done on right hind paws from control (non-immunized DBA1 mice) and CIA mice at different disease stages (up to 10 weeks after 1st immunization). Sacrifice at 2 days after 1st immunization: CIA 18, control 6 2 weeks: CIA 15, control 6 3 weeks: CIA 14, control 6 4 weeks: CIA 16, control 5 6 weeks: CIA 17, control 5 7 weeks: CIA 17, control 5 8 weeks: CIA 16, control 5 10 weeks: CIA 15, control 5