Sample data for analysis of sequence variation in HIV
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https://zenodo.org/record/3519267
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资源简介:
These are downsampled interleaved paired fastq datasets from Jair et. 2019 (https://doi.org/10.1371/journal.pone.0214820). The datasets were prepared by:
Downloading original data from NCBI SRA (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA517147)
Trimming contaminating Nextera adapters using trim-galore
Mapping reads against nxb2 reference of HIV genome (K03455.1) with BWA MEM
Restricting mapped reads to pol gene vicinity (K03455.1:2000-5100)
Downsampling mapped data to ~10% of the original with Picard's DownsampleSam
Converting BAM to Interleaved Fastq with Picard's SamToFastq
Gzipping resultant interleaved paired fastq files
创建时间:
2022-08-04



