Dexamethasone inhibits Mycobacterium tuberculosis-induced glycolysis but preserves antimicrobial function in primary human macrophages.

This data belongs to the figures from Thong et al 2025 Scientific Reports (Dexamethasone inhibits Mycobacterium tuberculosis-induced glycolysis but preserves antimicrobial function in primary human macrophages). The data is presented in a single excel file. Each tab in the excel file represents a different figure from the above paper. 
It is highly recommended to read the excel file in conjunction with each figure within the associated paper. The data has been organised based on the layout of figures in the associated Scientific Reports paper. 
This data consists of human monocyte derived macrophages and human alveolar macrophages. Human macrophages were left untreated or treated with vehicle control (ethanol, 0.02%) or dexamethasone (0.05-5 μM, as indicated). To model pathways of cellular death, MDM were treated with staurosporine (1 μM) for 24 h. The effects of dexamethasone on macrophage metabolism was examined by Seahorse metabolic flux analysis using the glycol..., Human Macrophage Culture
Buffy coats were obtained with consent from healthy donors (aged between 18–69; ethical approval, School of Medicine Research Ethics Committee, Trinity College Dublin). Peripheral blood mononuclear cells (PBMC) were isolated by density-gradient centrifugation over Lymphoprep (StemCell Technologies). Cells were resuspended in RPMI (Gibco) supplemented with 10% AB human serum (Merck) and plated onto non-treated tissue culture plates (Costar) for 7 days. Non-adherent cells were removed by washing every 2-3 days. Cultures were >90% pure based on co-expression of CD14 and CD68.
Human AM were retrieved at bronchoscopy, (ethical approval, the SJH/TUH Joint Research Ethics Committee). AM were plated in RPMI (Gibco) supplemented with 10% FBS (Gibco), fungizone (2.5 μg/ml; Gibco) and cefotaxime (50 μg/ml; Melford Biolaboratories). Cells were incubated for 24 h before washing to remove non-adherent cells. Adherent cells (predominantly AM) were used for experiments.
Macr..., # Dexamethasone inhibits Mycobacterium tuberculosis-induced glycolysis but preserves antimicrobial function in primary human macrophages.

Dataset DOI: [10.5061/dryad.rxwdbrvp0](10.5061/dryad.rxwdbrvp0)

## Description of the data and file structure

**Description:** Data for figures presented in *Dexamethasone inhibits Mycobacterium tuberculosis-induced glycolysis but preserves antimicrobial function in primary human macrophages. Thong et al., 2025 Scientific Reports.*

**The data is presented in a single excel file.**

### Files and variables

#### File: Thong_2025_Sci_Reports_Dryad_File.xlsx

##### Variables

* Cell type (either human monocyte derived macrophage (hMDM) or human airway macrophage (hAM)) is listed in box 1A of every data set within each excel sheet.

**Excel Sheet 1 (Figure 1-hMDM)**: Data is derived from human monocyte derived macrophages (MDM) that were treated with vehicle control (VC; ethanol 0.02%) or dexamethasone for 24 h prior to analysis in the Seahorse XFe2..., All individuals provided written informed consent and were made aware that collective results arising from samples given would be published. Each donor was assigned an anonymized, sequential identification number (starting at 1) after data analysis and graphing. These identifiers are arbitrary and cannot be linked to any personal information, ensuring complete anonymisation.