Transcriptome Analysis Reveals a Promotion of Carotenoid Production by Copper Ions in Recombinant Saccharomyces cerevisiae

When using YPM media, the carotenoid yield was increased 10-fold compared to using the YPD media. To elucidate the hidden mechanism, the transcriptome analysis was performed and showed that 464 genes changed significantly in YPM media. Furthermore, inspired by the differential gene expression analysis which indicated that ADY2, HES1, and CUP1 showed the most remarkable changes, we found that the improvement of carotenoid accumulation in YPM media was mainly due to the copper, since supplementation of 80 µM CuSO4 in YPD media could increase carotenoid yield 9.2-fold与使用YPD培养基（YPD media）相比，采用YPM培养基（YPM media）时，类胡萝卜素产量提升10倍。为阐明其潜在作用机制，本研究开展了转录组分析，结果显示YPM培养基中共有464个基因发生显著表达变化。此外，基于差异基因表达分析结果（其中ADY2、HES1与CUP1的表达变化最为显著），本研究发现YPM培养基中类胡萝卜素积累量提升的核心诱因在于铜离子；具体而言，在YPD培养基中添加80 μM硫酸铜（CuSO4）即可使类胡萝卜素产量提升9.2倍。