Effect of the application of the formulation of Pseudomonas chlororaphis PCL1606 in the prokaryotic and eukaryotic communities present in soil and rhizosphere samples taken from avocado plants inoculated with R. necatrix

In this work, a semi-commercial formulated product based on PcPCL1606 was used to study the impact of the application of PcPCL1606 on natural microbial populations with R. necatrix inoculation. The treatment was a preventive application (PcPCL1606 preventive), consisting of a single application with a semi-commercial formulate of PcPCL1606 (PcPCL1606 preventive) performed 50 days before inoculation with R. necatrix. To test the effect of the preventive PcPCL1606 application during biocontrol, samples from T3 of assay 2 (80 days after inoculating the fungal pathogen) in the control and preventive treatment where R. necatrix was inoculated were analysed. DNA was extracted from each of the 3 independent composite samples where formulated PcPCL1606 was applied (PcPCL1606 preventive) and 3 composite samples where it was not applied (Control). DNA samples taken from each rhizosphere/soil type were sent to be sequenced by ChunLab (Seoul, Korea) to obtain the microbial DNA sequences of the 16S rRNA gene and ITS hypervariable regions.