RNA Surveillance by the RNA Helicase MTR4 Determines Volume of Mouse Oocytes [day 14 total_RNA-seq-Nu_Cy]

Exosome is the main RNA surveillance and degradation pathway during oocyte growth. To figure out specific roles of RNA surveillance in oocyte growth, the pathway was destroied by deleting MTR4, the only known RNA helicase in exosome pathway. Total RNA-seq was performed using samples after nucleus-cytoplasm separation to examine the global subcellular transcriptional changes. Overall design: The total RNA-seq was performed with 10 nuclei or cytoplasms per sample. WT and MTR4-null oocytes were extracted and then nucleus-cytoplasm separation were performed with micromanipulation. Duplicates were conducted. Each sample was sequenced 5G.