Short term effect (0–4 h) of the AMPK inhibition by compound C (CC) in boar spermatozoa motility parameters in TBM.

Spermatozoa were incubated in TBM at 38.5°C in the presence or absence of the AMPK inhibitor, CC, (30 µM) during 4 hours. Samples measured at 17°C were considered as time 0. Spermatozoa motility parameters including straight-line velocity (VSL, expressed in µm/s) and coefficients LIN (Linearity coefficient in %), STR (Straightness coefficient in %), WOB (Wobble coefficient in %), ALH (Amplitude of lateral head displacement in µm) and BCF (Beat cross frequency in Hz) were measured by the ISAS system. This experiment was performed at least 6 times and results express the mean ± standard error of the mean. Statistical differences were considered when p