Internal Conductance to CO(2) Diffusion and C(18)OO Discrimination in C(3) Leaves

(18)O discrimination in CO(2) stems from the oxygen exchange between (18)O-enriched water and CO(2) in the chloroplast, a process catalyzed by carbonic anhydrase (CA). A proportion of this (18)O-labeled CO(2) escapes back to the atmosphere, resulting in an effective discrimination against C(18)OO during photosynthesis (Δ(18)O). By constraining the δ(18)O of chloroplast water (δ(e)) by analysis of transpired water and the extent of CO(2)-H(2)O isotopic equilibrium (θ(eq)) by measurements of CA activity (θ(eq) = 0.75–1.0 for tobacco, soybean, and oak), we could apply measured Δ(18)O in a leaf cuvette attached to a mass spectrometer to derive the CO(2) concentration at the physical limit of CA activity, i.e. the chloroplast surface (c(cs)). From the CO(2) drawdown sequence between stomatal cavities from gas exchange (c(i)), from Δ(18)O (c(cs)), and at Rubisco sites from Δ(13)C (c(c)), the internal CO(2) conductance (g(i)) was partitioned into cell wall (g(w)) and chloroplast (g(ch)) components. The results indicated that g(ch) is variable (0.42–1.13 mol m(−2) s(−1)) and proportional to CA activity. We suggest that the influence of CA activity on the CO(2) assimilation rate should be important mainly in plants with low internal conductances.