Fig5

(A) Quantitative real-time PCR analysis of Nox2 mRNA expression in Rad9+/+ and Rad9-/- MES cells exposed to 1G or SMG condition for 1 or 5days. The expression levels of Nox2 were normalized to the endogenous control GAPDH expression. (B) Quantitative real-time PCR analysis of Nox4 mRNA expression in Rad9+/+ and Rad9-/- MES cells exposed to 1G or SMG condition for 1 or 5days. The expression levels of Nox4 were normalized to the endogenous control GAPDH expression. (D) Quantitative comparison of Nox2 expression. Data were derived from three independent experiments . The expression levels of Nox2 protein were normalized to the endogenous control GAPDH protein expression.