Subunit vaccine elicited lung TRM cells use fibroblast IL-17R signaling to provide serotype independent immunity against hypervirulent K. pneumoniae

Subunit vaccine conferred lung protection against heterologous strain through the augmentaion of lung tissue resident memmory CD4+ T-cells. Overall design: ?We immunized WT C57BL/6 mice with OmpX+LTA1 or heat killed Klebsiella pneumoniae (ATCC 43816) twice 3 weeks apart, and 1 more week later we euthanized and removed the lung for single cell suspensions in addition to naive spleen cells. ?Our data had indicated that 24 hours post pulmonary infection of K1 strain at 10e4 CFU reveals quite higher bacterial burden in Il17raDermoCre postivie mice than in Il17raDermoCre negative mice, and therefore we harvested lung tissues to obatin RNA for bulk RNAseq at 4 hours post infection because any differences in gene expression would not be due to differences in lung bacterial burdens.