Natural variation in the atypical resistance gene Ptr confers broad-spectrum neck blast resistance in rice
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Supplementary tables for "Natural variation in the atypical resistance gene Ptr confers broad-spectrum neck blast resistance in rice" Table S1. Neck blast reactions of the 335 GWAS accessions inoculated with M64-1-3-9-1. Values represent mean values of DI=disease incidence (%); DSI=disease severity index; LL=lesion length (mm).*Values represent lesion length (mm) and are classified as resistant (R, 0–7 mm), moderately resistant (MR, >7–30 mm), moderately susceptible (MS, >30–55 mm), or susceptible (S, >55 mm). nd=no data. Table S2. Neck blast reactions of IRRI elite breeding lines inoculated with M64-1-3-9-1. Values represent lesion length (mm) and are classified as resistant (R, 0–7 mm), moderately resistant (MR, >7–30 mm), moderately susceptible (MS, >30–55 mm), or susceptible (S, >55 mm). Table S3. Modified Evaluation scale for neck blast disease (IRRI Plant Pathology Laboratory, 2019). Table S4. Primers used in the qRT-PCR in the Pi-ta and Ptr CO39 near-isogenic lines analysis and sgRNA off-target sequencing in CRISPR-Cas9 mutants. Table S5. Subpopulation-level neck blast reactions of the 335 GWAS accessions inoculated with M64-1-3-9-1 and the distribution of peak SNPs haplotypes. DI=disease incidence (%); DSI=disease severity index; LL=lesion length (mm). *Values indicate number of accessions in the 3K RGP. Table S6. SNPs exceeding the Bonferroni-corrected genome-wide significance threshold. Table S7. SNPs located within the 42.3-kb LD block on chromosome 12 (10.82–10.86 Mb) identified using Chr12:10,833,400 as the focal marker. DI=disease incidence (%); DSI=disease severity index; LL=lesion length (mm). Table S8. Corresponding SNP 10,833,400 alleles and Ptr alleles among the GWAS accessions used in this study. Values represent mean values of DI=disease incidence (%); DSI=disease severity index; LL=lesion length (mm). Table S9. Neck blast reactions of the Ptr and Pi-ta 25 LTH monogenic lines inoculated with the 20 Philippine standard blast isolates. Values represent lesion length (mm) and are classified as resistant (R, 0–7 mm), moderately resistant (MR, >7–30 mm), moderately susceptible (MS, >30–55 mm), or susceptible (S, >55 mm). LTH was included as susceptible check. Table S10. Neck and leaf blast reactions of the monogenic line harboring Ptra. The line was inoculated with the 20 Philippine standard blast isolates. Values represent lesion length (mm) and are classified as resistant (R, 0–7 mm), moderately resistant (MR, >7–30 mm), moderately susceptible (MS, >30–55 mm), or susceptible (S, >55 mm). LTH was included as susceptible check for neck and leaf blast. ND = No data



