Mucosal miRNA expression profiling in ileum of patients with Crohn's disease

In this study, we investigated miRNA expression profiles in ileal mucosa from CD patients in different settings (post-operative recurrent (POR) CD, newly diagnosed CD and late stage CD)) and controls. Ileal mucosal biopsies were obtained during endoscopy from 35 CD (20 POR (Rutgeerts score ≥i2b), 8 new and 7 late) patients and 8 normal controls. Total RNA, including small RNA, was isolated from ileal mucosal biopsies, labelled and hybridized to Affymetrix Genechip miRNA 2.0 Arrays.