Cell Surface Protein mRNAs Show Differential Transcription in Pyramidal and Fast-Spiking Cells as Revealed by Single-Cell Sequencing

We performed physiological recording and single cell RNAseq on mouse prefrontal cortical pyramidal and fast-spiking interneurons in the live brain slice preparation. One of the goals was to identify potential gene targets whose function might be disrupted in schizophrenia. Brain slices from mouse brain were acutely cultured, individual neurons recorded from and the RNA isolated from these same neurons for RNAseq analysis. The RNA was amplified with the aRNA procedure.