A high-throughput mutational scan of an acidic transcriptional activation domain

NIAID Data Ecosystem2026-03-10 收录

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Raw data for Staller et al. 2018 A high-throughput mutational scan of an acidic transcriptional activation domain Max V. Staller1, 2, Alex S. Holehouse3,4, Devjanee Swain-Lenz1,2,5, Rahul K. Das3,4,6, Rohit V. Pappu3,4, and Barak A. Cohen1, 2, * 1Edison Family Center for Genome Sciences and Systems Biology, Washington University in St. Louis School of Medicine, Saint Louis, MO, 63110 2Department of Genetics, Washington University in St. Louis School of Medicine, Saint Louis, MO, 63110 3Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO, 63130 4Center for Biological Systems Engineering, Washington University in St. Louis, Saint Louis, MO, 63130 5Present address: Department of Biology, Duke University, Durham, NC, 27708 6Present address: GNS Healthcare Inc., Cambridge, MA, 02139 * Corresponding Author and Lead contact: cohen@wustl.edu Files include: raw sequencing reads for replicate 1 (Sort 11) raw sequencing reads for replicate 2 (Sort 12 ) raw sequencing reads for replicate 1 of amino acid starvation condition (Sort 14) for calculating induction raw sequencing reads for mCherry only sorting (sort 15A) File of designed mutants (DNA sequence) (DNA_Seqs_GCN4_Array.txt). raw sequencing reads for paired end sequencing to look for mutations in the library (Sort11AD-BC R1 and R2) Key to barcodes: Sort_Bin 5’ inline barcode 3’ adaptor barcode S11_1 GCTCGAT IND71 S11_2 TAGACTAT IND71 S11_3 CGCTACCCT IND71 S11_4 ATAGTGGACA IND71 S11_5 GCTCGAT IND72 S11_6 TAGACTAT IND72 S11_7 CGCTACCCT IND72 S11_8 ATAGTGGACA IND72 S12_1 GCTCGAT IND69 S12_2 TAGACTAT IND69 S12_3 CGCTACCCT IND69 S12_4 ATAGTGGACA IND69 S12_5 GCTCGAT IND70 S12_6 TAGACTAT IND70 S12_7 CGCTACCCT IND70 S12_8 ATAGTGGACA IND70 S14_1 GCTCGAT IND69 S14_2 TAGACTAT IND69 S14_3 CGCTACCCT IND69 S14_4 ATAGTGGACA IND69 S14_5 GCTCGAT IND70 S14_6 TAGACTAT IND70 S14_7 CGCTACCCT IND70 S14_8 ATAGTGGACA IND70 S15_1 GCTCGAT IND69 S15_2 TAGACTAT IND69 S15_3 CGCTACCCT IND69 S15_4 ATAGTGGACA IND69 S15_5 GCTCGAT IND70 S15_6 TAGACTAT IND70 S15_7 CGCTACCCT IND70 S15_8 ATAGTGGACA IND70

创建时间：

2018-01-12