Effect of Gsdmc knockdown in murine KPC cells transcriptome

We identified that Gsdmc gene is important for pancreatic cancer in tumorigenesis and metastasis. Here we used KPC mice (LSL-KrasG12D/+; p53f/f; Pdx1-Cre)-derived cancer cells, KPC cells in short, to check the effects of Gsdmc knockdown on primary tumor growth and metastasis. The results suggested that Gsdmc knockdown inhibited the expression of key genes in multiple pathways, including cell migration, epithelial-mesenchymal transition, immune cell recruitment, and so on. The data deposited here provides the transcriptomic alteration of KPC cells after Gsdmc knockdown with two shRNAs. KPC cells named CHX2000 was isolated from KPC tumors and cultured as stable cell line. Two shRNAs targeting Gsdmc gene were transfected to CHX2000 cells and produce CHX2000-shGC1 and CHX2000-shGC2 cells.