Differentially Expressed Genes (DEGs) in floral bud transcriptomes
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The relative abundance for each transcript and unigene was quantified in the floral bud samples (S1, flowering induction; S2-S4, flower whorls development and D, dormancy) by aligning the reads to the transcriptome assembly using RSEM v1.3.0 software. To identify the differentially expressed genes the Deseq2 1.12.4 software was used. The Summary of DEGs.xlsx archive contains: the "Tables sheet" with tables about the number of DEGs (up- and down-regulated) in sucessive pairwise comparisons of floral buds, GO summary and the number and percentaje of DEGs codifying transcription factors; the "Gene Annotation sheet" with the annotation of DEGs; the Gene Ontology sheet" with the GO terms found for each transcript; the "KEGG sheet" with the KEGG terms found for each transcript; the "Transcription Factor Families" sheet with the description of DEGs codifying transcription factors.The DEGs.fasta archive contains the deduced nucleotide sequences of DEG transcripts.The diff.Expr.matrix... archive contains a heatmap showing the log2 of fold-change of DEGs vs the samples analized (S1-S4 and D floral buds). Yellow and magenta colors represent up- and down-regulated genes, respectively. Scale, representing the signal values, is shown at the top of the figure.The "MADS-box transcripts found during floral bud development in P. avium cv. Bing”.xlsx archive includes the Trinity identification (Trinity #id), MADS-box gene identification (Gene ID, this work), Gene Subfamily, Genbank accession number considering only P. avium, mean value of Transcripts Per Million (TPM) in S1-S4 and Dormancy (D) buds, Translation (Complete/Partial) using TransDecoder, Diferential expression (DEG Yes/No) and if the MADS-box genes was analyzed by qPCR.
创建时间:
2020-01-21



