Transcriptome_of_the_MOE_in_different_mouse_strains

High-throughput RNA sequencing of the main olfactory epithelium in two mouse strains. With this study we aim to dissect the genetic contribution to the expression of the olfactory receptor genes from environmental factors. For this, we housed a single black6 mouse with a 129S5 litter and vice versa, and dissected the MOE of the black6 animal and one 129S5 animal at 10 weeks of age. Three biological replicates were obtained for each combination. RNA was extracted using the Qiagen RNeasy kit using standard protocols. All twelve RNA samples were multiplexed together and sequenced in four lanes on the Illumina HiSeq platform.