Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availability. Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availability

Degradation of most yeast mRNAs involves decapping by Dcp1/Dcp2. DEAD-box protein Dhh1 has been implicated as an activator of decapping, and as a translational repressor, but their functions in cells are incompletely understood. We have analyzed these questions by a combination of ribosome profiling, RNA-Seq, CAGE analysis of capped mRNAs. Overall design: This study includes total 24 samples, 6 for ribosome profiling and 6 for RNA-Seq derived from duplicates of WT, dcp2D and dcp2-EE. Separately, total RNA of WT, dcp2, and xrn1 in duplicates, were subjected to CAGE-Seq (6 samples) and total RNA-Seq (6 samples). All strains were grown in YPD at 30ºC till the OD600 ~0.6