Additional file 10: Table S3. of Reversing chromatin accessibility differences that distinguish homologous mitotic metaphase chromosomes

GVF measurements of BAC control FISH probe signal co-hybridized with scFISH probes. Columns labelled A and B represent integrated intensity measurements for each homolog in a diploid metaphase cell. Normalized integrated intensities were obtained by taking the difference in integrated intensities between homologs, and dividing by the sum of the intensities of both homologs in a given cell. Slides 1 (0.25 μM ICRF-193) and 2 (0.10 μM ICRF-193) were processed in parallel and co-hybridized with BAC control probe RP11-57P14 and scFISH probe CACNA1B with DA. Irrespective of whether ICRF-193 reversed DA (slide 1, 0.25 uM ICRF) or showed limited effect (slide 2), control probes hybridized with consistently bright intensities to both homologs. No significant differences (p = 0.85) were observed in normalized integrated intensities among control probes between slides 1 (Δμ = 0.147, Median = 0.156) and 2 (Δμ = 0.156, Median = 0.123). Slides 3 (0.50 uM ICRF) and 4 (0.25 uM ICRF) were processed in separate hybridizations and co-hybridized with BAC control probe RP11-467L20 and scFISH probe RGS7 with DA. Similarly, no significant differences (p = 0.12) were observed in normalized integrated intensities among control probes between slides 3 (Δμ = 0.104, Median = 0.078) and 4 (Δμ = 0.162, Median = 0.110).